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Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

What fluorescent dye can be used in fixed cells for membrane imaging?

07 January 2021 4,802 2 View

QUANTUM INFORMATION: Why a Toffoli gate can be used to obtain any permutation gate?

Hi, i am sudying some quantum computer science and I am really struggling to find an explanation to the question done above. Any kind of help would be wonderfull. Greets.

09 November 2020 4,657 3 View

How to dye the spider gland of a spider to see in fluorescence (488nm)?

I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to...

14 October 2020 3,986 10 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

Why doesn't my dbRDA show all the environmental variables considered?

I am performing a db-RDA in R using the package Vegan and the function capscale. I have taken into account 24 environmental factors but the program only considerate 7 of this factors. I have 54...

26 August 2020 919 6 View

How should I present microRNA expression data from a single sample (leaves)?

Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...

30 July 2020 9,696 3 View

Additionally, to GBIF database, Where I can find global records of Aedes sp. and Anopheles sp.?

Hi I'm interested in to know where find records of Aedes sp. and Anopheles sp. I visited GBIF repository but I want to know other sources of data

10 June 2020 2,535 3 View

What is the best, easiest and fastest method to construct a bacterial genomic DNA Library? Any other alternative instead fosmid kit?

I would like to clone a big region (50kb) of DNA.

08 April 2020 5,807 5 View

Murine alpha-synuclein overexpression in PD mouse model?

I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...

26 March 2020 4,022 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View