Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

HowScholarships, Fellowships and grants in Materials engineering for international students to study abroad?

On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...

10 December 2020 3,686 3 View

Merging two same papers in a one paper?

Hello, I have three same papers which have different numbers of citations and different number of readings and I would like to merge these three papers in one paper with taking account of a new...

20 September 2020 5,275 6 View

Does polysome profiling in 4mL tube instead of 12 mL needs a scale down in ultracentrifugation time ?

Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...

19 September 2020 8,717 10 View

What is the sensitivity of SHM technique based on electromechanical impedance method to identify a crack in a concrete beam?

The sensitivity of the technique is important to know how to identify the appearance of cracks

17 August 2020 4,156 9 View

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31 May 2020 6,077 6 View

How can you efficiently collect a ground-nesting bee when it is inside its nest?

I am looking for an efficient way to collect a ground-nesting bee at its nest, so that when encountering a ground-nesting bee nest, I could determine what species created that nest. Ideally, I was...

21 February 2020 5,269 3 View

Does someone has an interest for XIXth century herbaria prepared in Brazil?

While investigating about French women botanists in the XIXth Century I chanced upon two herbarias, made from plants collected in Brazil. I am looking for someone who could help to evaluate the...

07 October 2019 2,560 6 View

Journals to submit a method to assess Vulnerability of Hotels to Earthquakes ?

Dear all, What are the most appropriate journals to submit a method we developed to estimate the seismic vulnerability index for touristic accommodations ? and its application to a pilot zone of...

08 September 2019 8,878 3 View

The "4p1000" initiative on soil carbon storage: Should the name be changed?

At the COP 21, in France, a few years back, an initiative was proposed to increase the carbon content of soils by 0.4% or 4 per 1000 per year. The claim was made that, in so doing, soils could...

28 May 2019 3,080 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View