I would like information on a methods for phytochelatins determination with HPLC and without the use of the additional step of derivatization and without MS, if possible.
Following paper will help you out.
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
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Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...
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I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...
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I’m looking for an inclusive leadership scale to include in a mediation analysis with psych safety and workplace belonging, what would you use
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23 April 2024 6,534 0 View
This question is directed on the performance of the Sysmex DI-60 digital morphology analyzer and its contributions on the improvement in the laboratory workflow. I am curious as to what...
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It is known that self-medication with antibiotics is an arising problem, becoming a major constituent to antibiotic resistance. Moreover, self-medication heightens the possibility of developing...
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Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...
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A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
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Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
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I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
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Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View