Dear Sir. Concerning your issue about the chemical assay to detect fluor . Fluorescent assays are widely used in High throughput Screening (HTS) and direct fluorescence, coupled assays, fluorescence polarization, TR-FRET and DSF will be discussed. A common type of assay includes the consumption or generation of a fluorescent molecule. An example would be IDH1 (isocitrate dehydrogenase 1, an enzyme in the citric acid (TCA) cycle), which utilizes NADP and generates the fluorescent product NADPH, an example of an endogenous fluorophore. If the substrates or products of the enzyme reaction of interest are not endogenously fluorescent, a pro-fluorescent substrate can be used that only becomes fluorescent upon enzyme activity. For example, Ubiquitin-rhodamine is frequently used to assess the activity of deubiquitinases, which cleave off the Rhodamine fluorophore leading to an increase in fluorescence. These types of assays can be run in either endpoint or kinetic mode, which will be described in more detail below. Using a standard curve, the amount of fluorophore present at the end of the reaction can be calculated and used to design a counterassay that can detect both autofluorescence and quenchers. The amount of fluorescent substrate and/or products is plated in assay buffer and the effect of compound on the observed fluorescence is measured. For more details, I think the following below link may help you in your analysis:

https://www.ncbi.nlm.nih.gov/books/NBK343429/pdf/Bookshelf_NBK343429.pdf

http://pubs.acs.org/doi/abs/10.1021/ac902526g%40proofing

Thanks

What do you mean by " fluor"? Fluorescence? An Alexa Fluor dye?

HPLC can be used to look for a specific compound; anything that fluoresces has to absorb light, so UV detection will work.

If you mean fluoride, there are fast liquid test (e.g. Spectroquant Merck) to determine fluoride in solution.

if it is ion F-, you can measure easily. 2 methods that I used to use a lot are: ion chromatography (this uses an anion exchange chromatography column, after sample goes through the column, different ions get separated into different zones and get detected with an electrical conductivity detector). Another method is capillary electrophoresis, also using an electrical conductivity detector. You can maybe have a look here in one of my paper:

Conference Paper Capillary electrophoresis with contactless conductivity dete...

Thank you for your helpful answers.

Regards Dorinda