Do you have the specific conditions (concentrations and volumes) for PCR with ITS used in this article?

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Is defatted soybean considered a pulse?

According to various research, soybean is not considered a pulse because of its high fat content.

30 June 2024 6,311 0 View

How can I reassgn my expertise in ResearchGate?

I inadvertently 'accepted' a specialisation in biology, but it was the wrong Richard McCullagh. I'm in Australia and he is in the USA. Can I delete that reference and enter 'philosophy of...

23 June 2024 3,124 0 View

How do I remove the research lab affiliations?

Some time ago I opened the research lab attachment and it populated automatically with all co-authors who were not members of another research lab. This has turned out to be embarrassing for me...

12 May 2024 6,275 0 View

How build Forest plot in STATA?

(STATA) Hello everybody. I would like to build a Forest Plot graph that includes all this information from the table. I am unable to build the commands to include this information in the chart....

11 May 2024 6,944 6 View

What is my Repository Id in ResearchGate?

What is my Repository ID number?

29 April 2024 9,014 0 View

How to use incomplete stage-specific counts to estimate status and trend?

I'm wondering how one handles demographic data that are incomplete to estimate status and trend. For example, consider a 5-stage plant population with stages 1) Seed bank, 2) Seedlings 3) Juvenile...

19 April 2024 615 3 View

I am looking for L&D professionals to participate in a qualitative Delphi research study on Virtual Communities of Practice (VCoPs)?

Dear Professionals in Learning and Development, I am pleased to invite you to participate in a significant doctoral research study at University of Arizona Global Campus, dedicated to exploring...

12 April 2024 4,507 0 View

I am looking for L&D professionals to participate in a qualitative Delphi research study on Virtual Communities of Practice (VCoPs)?

Dear Professionals in Learning and Development, I am pleased to invite you to participate in a significant doctoral research study at University of Arizona Global Campus, dedicated to exploring...

06 April 2024 8,099 3 View

Can I make public publications by Elsevier that are over two years old?

non tech question

17 March 2024 4,861 5 View

Can I use social media to obtain a non probability sample of a country's labour force (n=400) for PhD research?

I am seeking to examine the relationship between variables in the labour force of a small country.

01 March 2024 3,417 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View