Do you have the specific conditions (concentrations and volumes) for PCR with ITS used in this article?

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What is the recommended/optimal time for resting mouse splenocytes after thawing?

This is my first time working with mouse cells. We isolated and froze down in 90% FCS and DMSO. I would like to retrieve them and do immunophenotyping and intracellular cytokine staining

03 January 2021 6,399 1 View

Can CMOS and CCD images sensors be used as microphones?

I was thinking about Msps and Gsps image sensors in different environments. If a pair of identical image sensors - one exposed to air, gases, plasmas, or partial vacuum - and one in a good...

21 December 2020 9,747 2 View

Is there a tool to find the closest species with an assembled genome for any given species?

As more and more genomes are being sequenced the likelihood of having a close relative being sequenced is rising. So lets say I have a non-model species Chela cachius and I want to find the...

25 November 2020 6,267 4 View

Where can I find a longterm global mean EVI/NDVI dataset?

I am looking for a longterm (10+ years) dataset of mean EVI/NDVI or annual maximum EVI/NDVI and similar. Basically, I am trying to avoid downloading tons of images and doing the averaging myself,...

28 September 2020 8,374 2 View

What would be the criteria for an expression vector designed for S. cerevisiae to also work in S. pombe?

I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...

18 September 2020 7,460 2 View

Can we infuse drug along with sedative?

I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...

15 September 2020 7,501 5 View

What is a suitable solvent for extraction of arsenic?

Hi. I'm doing some work on arsenic speciation. I am following a paper titled, "SPECIATION OF ARSENIC IN A CONTAMINATED SOIL BY SOLVENT EXTRACTION." which calls for the use of chloroform or benzene...

06 September 2020 6,351 4 View

Ideal saline volume for intravenous injection in rats ?

Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...

27 August 2020 9,444 2 View

Does mysticism have a scientific basis?

Mysticism is often treated as the opposite of science. But is it? Please see Natural Laws and Mysticism in the Cosmic Cycle of Creation

25 July 2020 6,696 80 View

Which molecular dynamic simulation software is more applicable for protein and large-area surface interaction simulation? And how to run it?

Dear experts: I am trying to do the molecular dynamic simulation for protein-surface interaction, the surface (like cellulose or PET) is a film-like or crystalline material with large surface...

20 March 2020 3,960 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View