How to name a strain of a fungus you isolate?

I isolated a wood-decaying fungus that I would like to test. I know the species already, but I don't know what should I do about the strain. Are there any guidelines to know how to name this...

02 March 2019 3,338 3 View

Have you use Gateway system to express proteins?

06 July 2018 7,330 2 View

Which is the best media to grow "Amyloporia spp."?

31 December 2017 7,129 5 View

Who, how and based on which criteria are selected the author and/or subject for the Annual Review of Phytopathology, or Microbiology?

There are ways to nominate scientist that deserve that opportunity?

09 October 2017 5,133 3 View

Any online free-access repository for old scientific papers?

Hello everyone, I was just wondering if anyone knows if there is any online "repository" or databank where I can find really old scientific papers. I feel this could be a incredibly useful tool...

09 October 2017 4,073 4 View

How different can cultures of the same fungi but different strains look like?

04 May 2017 6,759 24 View

Is there any statistical test/procedure to measure relationships among a vector (x, y, z), as explained variable, and multiple explanatory variables?

I have carried a survey to consumers. A question can be seen as a vector (x, y, z) with x+y+z=1. For example, for a consumer I have: x=0.5, y=0.3, z=0.2. Is there any statistical test/procedure to...

04 May 2017 1,292 16 View

Can your nationality contribute to the end of your scientific career?

11 December 2016 6,758 1 View

Do you have DNA from plants affected by phytoplasmas of groups 16SrII, 16SrIII, 16SrVI, 16SrIX, 16SrXI, or 16SrXIV? At least one of those?

09 October 2016 6,161 3 View

How to stirilize tannins?

08 September 2016 9,335 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View