For phenolics you can use A=0.1%H3PO4, B=ACN, 280nm, Flow=1ml/min in gradient mode. There are so many papers in literature, u can use following references:
1. Phytochem Anal. 2013 Sep-Oct;24(5):504-12. doi: 10.1002/pca.2450. Epub 2013 Jun 28. Characterisation of phenolic compounds by HPLC-TOF/IT/MS in buds and open flowers of 'Chemlali' olive cultivar.Taamalli A, Abaza L, Arráez Román D, Segura Carretero A, Fernández Gutiérrez A, Zarrouk M, Nabil
2. LC/MS/MS Characterization of Phenolic Constituents in Dried Plums. Nianbai Fang ,* Shanggong Yu , and Ronald L. Prior
Arkansas Children's Nutrition Center, 1120 South Marshall Street, Little Rock, Arkansas 72202
As usual you'll always be the first to comment because phenolics are truly your expertise! Thank you for these Dr. Joshi! Do you have an available HPLC-MS/MS in your institution? Are you open for collaboration?