What software/program you recommend to convert fasta file to Nexus.nxs?

I need to convert my fasta format alignment file to "Nexus.nxs" to be able to use it in Mybeys. What is your suggestion? So far the files I created using Mega x as well as an online database...

09 October 2019 449 1 View

how to export a phylogenetic tree into word document using MEGA X?

I have built a tree of my interest but MEGA X displays an error when saving the image as PDF file! I was wondering how can i save/ copy the tree into a word file. Thanks all

09 October 2019 4,045 5 View

DO you recommend GC-MS/EI or CI?

I am gonna do some analyses / detection on plant secondary metabolites (Terpenes and mostly volatile ones), Was wondering which method might be more beneficial- GC-MS-EI or CI? In most recent...

09 October 2019 2,384 2 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

08 September 2019 4,083 3 View

Do I need to carbonize the EM formvar grids to visulize virus like particles?

Dear researchers, I am going to fix my virus like particle sample using negative staining. However, the grids i have are only formvar without carbon coated. Do you have any idea what difference it...

10 November 2018 5,842 7 View

Best method to resuspend and anneal oligose for downstream ligation?

Dear Researchers, I have ordered oligose which should be annealed together for cloning/ ligation experiment. They are 66 in length and stickiy ends using two different restriction enzymes. The MW...

06 July 2018 9,829 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

04 May 2018 1,635 2 View

Why my SDS PAGE get stuck in the middle of the gel and the last lanes of the ladder are not seperating properly?

Dear All, I recently have a problem with my SDS-PAGE running. After staining with Brilliant blue and de-staining, I see that the proteins have not been run until the end of the gel. I increased...

04 May 2018 4,267 13 View

Recommending a protocol for VLPs isolation from e.coli?

Hi all, Iam expresing a virus capsid protein in e.coli. I need an optimised protocol for isolation virus like particles from e.coli cells. Using ultarcentrifugation I have a protocol but usually...

04 May 2018 8,178 2 View

Does anyone know of a plant based-vaccine against HAV?

Hi all, I am looking for some papers/ publications where a Hepatitis A vaccine has been tried or made in any plant. To my knowledge, There are not that much study on plant based vaccine against...

01 February 2018 2,556 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View