What is the best concentration for initiating the ARPE19 / RPE in T-flask and N-well plate with DMEM-F12?

Dear friends, I am working on RPE (human/mice primary RPE and ARPE19) for a while. The seeded density is 100.000 cells/ml. For T-75 flask, 6-well plate I used 20 ml and 3 ml/well respectively. The...

31 December 2014 583 0 View

Some chemical for DNA staining, such as SYBR non-toxic for scientists?

We know that chemicals which can attach to DNA structure are toxic, although toxicity is very low. Therefore, I guess that some manufacturers give us inaccurate information when they says that...

31 December 2013 9,179 2 View

What is the best method to identify Kd (dissociation constant) and Ka (association or equilibrium constant)?

04 May 2013 6,605 4 View

What is the best kit or protocol for DNA genome extraction from blood sample?

I am conducting multiplex PCR experiments for infertility detection. And I want to collect DNA from blood sample. However, I do not what the best method for DNA extraction is. What is your experience?

02 March 2013 1,082 16 View

What is the best immunologic adjuvant for mice and rabbits?

There are many protocols with a lot of adjuvants such as aluminium salts, squalene, freunds, etc. Can you give me your experience with how to choose the most comfortable adjuvant for total and...

31 December 2012 7,014 8 View

What is the best microtitrate plate for direct-ELISA technique?

I am going to conduct ELISA assay, but I do not know what microtitration plate is best for high efficiency of protein fixation.

31 December 2012 5,846 12 View

What is the best technique to identify alternative slicing result in vivo and in vitro ?

There are many way arrange exon in the alternative slicing, so how to identify what exon and exon's order in the mature mRNA ???

11 December 2012 767 2 View

Do you have any advice on LED - UV transilluminator?

I am need to buy a new UV transilluminator. A company presented a new LED - UV transilluminator to me and I am interested in buying it, but don't have any experience with this instrument. Can you...

10 November 2012 7,806 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View