Dear all,
We have primary myeloma cells in RPMI + 20% FCS and we are trying to establish a cell line from it. Cells proliferate but viability is quite low (~40%), for this reason we have tried to add different concentrations of IL-6 and other growth factors. Also we tried different cell densities. Waste cell products seems to be quite high. Do you have any advice on how to improve viability of primary myeloma cells?
Hello
use the RPMI 1640 media supplemented with 10% FBS and penicillin (100 U/mL), and streptomycin (100 g/mL). In some experiments co-cultures were treated with various doses of bortezomib, dexamethasone and melphalan for 72 h and at the end of the incubation period, d-luciferin K+ salt was added to the cultures (150 μg/ml) . Also ,
the interleukin (IL)-6 or insulin-like growth factor-1] were insufficient to stimulate their long-term growth in vitro .
Look at links here
Good Luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748973/
https://www.hindawi.com/journals/bmri/2014/510408/
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