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Dear all, We have primary myeloma cells in RPMI + 20% FCS and we are trying to establish a cell line from it. Cells proliferate but viability is quite low (~40%), for this reason we have tried to...
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I am wondering if there is an effective transfection protocol, that increases the efficiency in this suspension cells. Any tips are welcome.
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We would like to freeze primary myeloma cells before testing the compounds. Does anyone have any experience with this? What cryomedia did you use? Any advice on thawing the cells?
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