I want to isolate and purify intracellular membrane (mitochondrial, endoplasmic reticulum, plasma membrane) from the peach fruit simultaneously. Does anybody have any protocol for these procedures?
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
Hi, Could someone explain the primary differences between deep-seated landslides and slope destabilization? In particular, definition and characteristics, mechanisms and triggering factors,...
02 August 2024 4,212 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions?
25 July 2024 5,208 2 View
give suggestions
22 July 2024 5,664 2 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...
19 July 2024 6,569 3 View
If I want to invent my own hypothesis testing method, where should I get started ?
15 July 2024 5,376 5 View
I am currently working on a research paper focused on the control of Connected and Autonomous Vehicles (CAVs) utilizing multi-agent reinforcement learning methods. At this stage, I am seeking a...
14 July 2024 2,620 2 View
I've earned 1 best paper award and 4 best oral presentation awards. What should I do next to elevate my academic capabilities to the next level ?
14 July 2024 6,071 5 View
I read several information security books. How do I start writing anti-virus softwares ?
13 July 2024 8,180 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View