Wang Ke

6 Questions 5 Answers 0 Followers

Questions related from Wang Ke

How to preform an identification of transgene in tomato fruit in an easy way ?

Hi, everyone. E8pro::pcambia-1300-CDS-YFP has been transformed into tomato (AC). Although the mRNA of YFP can be detected for first positive screening, but we want to detect if the CDS is...

15 November 2022 452 0 View

How to understand chilling-induced water deficit in detached horticultural products under chilling condition?

Freezing stress can induce water loss due to the fact that formed ice breaks the membrane and electrolyte flux out of cell. However, chilling stress can also cause water loss.several reports...

03 August 2016 4,388 4 View

How do I dissolve nordihydroguaiaretic acid (NDGA)?

I want to use NDGA solution to treat fruit. But it is insoluble in water, how to dissovle NDGA ? I have found it is soluble in ethonal, DMSO, but ethonal properity is harmful to fruit, and effect...

23 June 2015 1,087 2 View

How can I predict the importance of the RPKM value of members in a gene family(encoding an enzyme)?

C5h C10h C15h T5h T10h T15hgene1 1 2 3 30 32 29gene2 10 12 13 20 21 23gene3 100 103 105 200 205 208gene4 1000 1100 1200 1600 1600 1650 I got the RPKM value of members in a gene family (encoding an...

22 May 2015 8,471 3 View

Do you have a protocol for intracellular membrane isolation and purification?

I want to isolate and purify intracellular membrane (mitochondrial, endoplasmic reticulum, plasma membrane) from the peach fruit simultaneously. Does anybody have any protocol for these procedures?

12 March 2014 8,568 0 View

Which subcellular membrane markers should be chosen to test purity of plasma membrane by immunoblotting? where can I buy them?

I want to evaluate the purity of plasma membrane-enriched fractions isolated in our laboratory by immunoblotting with antibodies raised against known subcellular membrane (endoplasmic reticulum,...

18 December 2013 3,917 2 View