If I should add G418, do you know what is the final concentration I should add to the culture media?
One should always run a kill curve and test different concentrations as even sub-lines or sub-clones might differ in their sensitivity.
What potential and/or current corrections should be applied to perforated patch recording? What about liquid junction potential?
05 June 2014 8,198 3 View
I've seen a few works, but do you think it is possible to observe the activation of ClCa in whole cell configuration after ATP (that should increase intracell calcium), even if you're buffering...
03 April 2014 8,774 1 View
I've to perform patch clamp exps on CaCC after stimulation of purinergic receptors. I've seen both APT or UTP is used, which one is preferred? I know that ATP solutions are quite instable and ATP...
02 March 2014 2,406 16 View
Does anybody know if lysine (100 mM) added to the cell culture media has toxic effects?
05 June 2012 6,979 5 View
I'm going to use cells infected with HIV-based translentiviral recombinant vectors containing the human wt- or F508del-CFTR cDNAs. Does anyone know which biosafety level is required to use this...
04 May 2012 3,581 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View