What potential and/or current corrections should be applied to perforated patch recording?

What potential and/or current corrections should be applied to perforated patch recording? What about liquid junction potential?

05 June 2014 8,129 3 View

Do S9 and C38 cell lines have to be cultured at the presence of G418?

If I should add G418, do you know what is the final concentration I should add to the culture media?

05 June 2014 2,437 1 View

Can a calcium activated chloride channel be activated by ATP in whole cell configuration?

I've seen a few works, but do you think it is possible to observe the activation of ClCa in whole cell configuration after ATP (that should increase intracell calcium), even if you're buffering...

03 April 2014 8,709 1 View

Purinergic receptor stimulation?

I've to perform patch clamp exps on CaCC after stimulation of purinergic receptors. I've seen both APT or UTP is used, which one is preferred? I know that ATP solutions are quite instable and ATP...

02 March 2014 2,334 16 View

Safety of cells infected with HIV-based translentiviral recombinant vectors

I'm going to use cells infected with HIV-based translentiviral recombinant vectors containing the human wt- or F508del-CFTR cDNAs. Does anyone know which biosafety level is required to use this...

04 May 2012 3,505 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View