(Commercially available) plant extracts are produced in different ways. Which methods (probably) lead to extracts containing nucleic acids, especially microRNAs? Is there any paper dealing with this question?
Yes, plats do contain micro RNA. In fact there is a data base
http://bioinformatics.cau.edu.cn/PMRD/ I am pasting some example s
< 1 > Gustafson, A.M., Allen, E., Givan, S., Smith, D., Carrington, J.C. and Kasschau, K.D. (2005) ASRP: the Arabidopsis Small RNA Project Database. Nucleic acids research, 33, D637-640. [NCBI]
< 2 > Rajagopalan, R., Vaucheret, H., Trejo, J. and Bartel, D.P. (2006) A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes & development, 20, 3407-3425. [NCBI]
< 3 > Baulcombe, D. (2004) RNA silencing in plants. Nature, 431, 356-363. [NCBI]
< 4 > Li, A. and Mao, L. (2007) Evolution of plant microRNA gene families. Cell research, 17, 212-218. [NCBI]
< 5 > Rhoades, M.W., Reinhart, B.J., Lim, L.P., Burge, C.B., Bartel, B. and Bartel, D.P. (2002) Prediction of plant microRNA targets. Cell, 110, 513-520. [NCBI]
< 6 > Griffiths-Jones, S., Grocock, R.J., van Dongen, S., Bateman, A. and Enright, A.J. (2006) miRBase: microRNA sequences, targets and gene nomenclature. Nucleic acids research, 34, D140-144. [NCBI]
< 7 > Jones-Rhoades, M.W., Bartel, D.P. and Bartel, B. (2006) MicroRNAS and their regulatory roles in plants. Annual review of plant biology, 57, 19-53. [NCBI]
< 8 > Archak, S. and Nagaraju, J. (2007) Computational prediction of rice (Oryza sativa) miRNA targets. Genomics, proteomics & bioinformatics / Beijing Genomics Institute, 5, 196-206. [NCBI]
< 9 > Barakat, A., Wall, P.K., Diloreto, S., Depamphilis, C.W. and Carlson, J.E. (2007) Conservation and divergence of microRNAs in Populus. BMC genomics, 8, 481. [NCBI]
< 10 > Sunkar, R., Chinnusamy, V., Zhu, J. and Zhu, J.K. (2007) Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends in plant science, 12, 301-309. [NCBI]
< 11 > Sunkar, R., Zhou, X., Zheng, Y., Zhang, W. and Zhu, J.K. (2008) Identification of novel and candidate miRNAs in rice by high throughput sequencing. BMC plant biology, 8, 25. [NCBI]
< 12 > Yao, Y., Guo, G., Ni, Z., Sunkar, R., Du, J., Zhu, J.K. and Sun, Q. (2007) Cloning and characterization of microRNAs from wheat (Triticum aestivum L.). Genome biology, 8, R96. [NCBI]
< 13 > Zhou, X., Wang, G. and Zhang, W. (2007) UV-B responsive microRNA genes in Arabidopsis thaliana. Molecular systems biology, 3, 103. [NCBI]
< 14 > Lindow, M., Jacobsen, A., Nygaard, S., Mang, Y. and Krogh, A. (2007) Intragenomic matching reveals a huge potential for miRNA-mediated regulation in plants. PLoS computational biology, 3, e238. [NCBI]
< 15 > Jones-Rhoades, M.W. and Bartel, D.P. (2004) Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Molecular cell, 14, 787-799. [NCBI]
< 16 > Wang, J.F., Zhou, H., Chen, Y.Q., Luo, Q.J. and Qu, L.H. (2004) Identification of 20 microRNAs from Oryza sativa. Nucleic acids research, 32, 1688-1695. [NCBI]
< 17 > Chen, Z., Zhang, J., Kong, J., Li, S., Fu, Y., Li, S., Zhang, H., Li, Y. and Zhu, Y. (2006) Diversity of endogenous small non-coding RNAs in Oryza sativa. Genetica, 128, 21-31. [NCBI]
< 18 > Enright, A.J., John, B., Gaul, U., Tuschl, T., Sander, C. and Marks, D.S. (2003) MicroRNA targets in Drosophila. Genome biology, 5, R1. [NCBI]
< 19 > Hofacker, I.L., Fontana, W., Stadler, P.F., Bonhoeffer, L.S., Tacker, M. and Schuster, P. (2004) Fast folding and comparison of RNA secondary structures. Monatsh.Chem, 125, 167-188. [Linkage]
< 20 > Lee, R.C., Feinbaum, R.L. and Ambros, V. (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 75, 843-854. [NCBI]
Thanks for your help. But I did not mean the plant itself, but the plant extracts. When parts of a plant are treated with water, oil, alcohol or other solvents in order to create an extract (for a example a tea), do some of these extracts contain miRNAs?