Hi everyone!

I'm in the process of designing gRNA and template for a CRISPR mouseline project.

The aim is to insert a fluorescent reporter upstream of the N-terminus of a GPCR, separated by a P2A site. This approach will add at least one proline to the N-terminus of our receptor.

The receptor has an N-terminal signal peptide and I'm worried that the addition of one to a few amino acids might affect ER targeting and translocation.

Does anyone have experience with small modifications of N-terminal signal peptides?

Thank you in advance!

-Nadine