Do mold or dampness or the presence of both cause asthma or allergic responses?

More Hal Levin's questions See All

What statistical test to use to compare two groups pre/post treatment scores?

I have two groups (treatment/placebo). for each group i have patients with paired data (before and after treatment). Paired t test underlines a significant change for the after treatment (however...

30 October 2022 1,142 8 View

Can you reuse Kugelmeier 5D plates for cellular spheroid formation?

Hello everyone, I am wondering whether someone has reused Kugelmeier 5D plates for spheroid preparation in 3D-cell culture. So after the formation of spheroids washing it and sterilizing it again...

14 October 2021 3,327 0 View

Any embryonic system where we know the number of molecules of a specific ligand and the # of receptors?

Are there any developmental systems (Drosophila?) in which we know the actual # of ligand molecules, and # of receptors, for a specific developmental signal? I want to know if there are cases...

29 May 2019 7,395 2 View

What cell line for reporting stress?

Are there cell lines that either express a reporter of stress (broadly - not just one specific stress type) like a GFP driven by some stress promoter, or one that has an endogenous stress marker...

18 January 2019 7,055 8 View

Is it ok to prepare a RIPA buffer without EGTA?

Anyone knows if it's ok to prepare RIPA buffer without EGTA? My intention is to use this RIPA buffer to lysate cells for a western blot of b-catenin and phospho b-catenin. I have everything it's...

23 December 2018 347 3 View

Do you have recent, valid data on human metabolism at different activity levels?

I would like to have data on normal human activities and associated metabolic rates for use in estimation heat and CO2 emitted during various normal activities.

15 June 2016 399 4 View

Can somebody recommend literature in relation to evolving and diversification of foraging strategies in Cyprinidae?

Supposedly, basal foraging strategy in cyprinids is benthivory. Other more specialized mode of feedings (piscivory, planktivory, plant feeding, detritivory, scraping feeding, some else?) should...

10 September 2015 602 5 View

What are the best ways to find cortical sources of ERD/ERS?

I have a set of ERD/ERS data for different subjects groups and different conditions and would like to compare the source estimates for these reactions between groups. I initially expected to use...

18 August 2014 1,835 4 View

Can anyone help with a ds cDNA synthesis cloning problem?

Hi, I have been using Thermo Scientifics Maxima H Minus cDNA double stranded synthesis kit to generate dscDNA. I carried out 2 rxns with oDT and RH primers (10 pmol) and got ~10 kb band for oDT...

08 July 2014 6,414 14 View

Is someone interested in a database of over 360 published papers addressing indoor microbiome and environmental characteristics?

It is in MS Access format, enabling searches for any organism or environmental factor or substrate (air, dust, floors, walls, woods, gypsum board, etc.). We hope you find it useful and if you have...

20 February 2014 2,272 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Kuldeep Patel

Hi, If there is no problem with yield of DNA than go for gel/colum purification of DNA which will also get rid of contamination of phenol which hinders in down stream process. If you are getting three bands like undigested plasmid after precipitating is it possibility of self ligation. Instead of phenol pptn you can try simple Na-acetate pptn. Also check the color and pH of phenol, if there is a problem in phenol DNA will get degraded, and you will see smear of DNA on gel.

Sorry the above answer was not for this question.

Jay Siddharth

It has been worked on from various angles, but right now the mold variety and the genetics of the inhabitants including age factor are the main factors.