Hello Sandipan,

mitochondria are connected to actin filaments. This is important for targeting mitochondria to specific locations in the cell and the controlled division of these organelles. Here is a link to nice review: http://dx.doi.org/10.1016/j.ceb.2013.09.002

So I wouldn't worry too much about the presence of beta-actin in your mitochondrial preparation.

Best regards

Hi Christian thank you so much for answering :) Maybe I should try tubulin as a quality control marker

Hello Sandipan,

I had a similar problem did tubulin worked out?

Best Regards

Hadile Osama Kobeissy and Nadia, sorry for the delayed reply. Yes Tubulin worked pretty good as loading control of cytosolic fractions but it was not in mitochondrial fractions. Where as actin was present in the mitochondrial fraction. I was trying to separate mitochondrial fraction from cytosolic fraction and used tubulin as a marker for quality control but actin cannot be used for same purpose

Hello Sandipan Datta, I had tried recently, tubulin show up in both of the preparation of mitochondrial fraction and cytosolic fraction.

Tubulin worked fine for me. No band visible in my mitochondria isolation but clear B-actin in all.

I had actin too in my isolated mitochondria samples