My cells don't spread well on acid washed coverslips, I have no clue why? Do I need to coat them with laminin or fibronectin or anything else?
Are you trying to create mitotic spreads? If so try storing the slides in EtOH over night then air dry, put the slides in ice for 10-15 minutes prior to dropping your cells.
When I was going for fluorescence microscopy on glas slides (LabTek chamber), I was always using a 1:40 dilution of collagen with PBS for coating, then cells are attaching and growthing nice.
No Ian Cartwright, I am seeding cells for immunofluorescence microscopy and they look more rounded even after 24 hours of seeding. That's why I was wondering if they need some special coating for them to spread well. Thanks for your response though.
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