Please Tell your Suggestion about Targeted drug delivery for Breast cancer treatment.What is your Suggestion ?

I will refer for Do my Mpharm project on Targeted Drug delivery for the treatment of Breast cancer.Please tell tell your Suggestion,Opinion,Idea about Possibility ,merits and demerits. Please...

05 December 2020 5,582 4 View

Shall I submit the article simultaneously to another journal even after request to first journal to withdraw the manuscript???

I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...

06 August 2020 1,802 3 View

Trapped water inside reduced graphene oxide at room temperature, will this affect the electrochemical performance of suprcapacitor?

How trapped water inside the RGO sheets helps to enhance the specific capacitance of energy storage material?

02 January 2019 7,308 4 View

Can i use my PCR product as DNA?

I had done PCR with cDNA and got some non specific band. Now i have very limited cDNA, which is not enough to perform all PCR, So can i use my previous PCR product as DNA. I have attached my Gel...

06 September 2018 8,721 7 View

How to get a thick band of GAPDH?

I tried to find GAPDH from mice brain after anesthesia, Using Kit, I find RNA and then cDNA. However, I get the band but the thickness is not like that what I desired. So what to do to get a thick...

13 August 2018 9,501 5 View

Relation between DC gain and poles of a system?

What is the reason that when we minimize the dc gain of the asymptotically stable LTI system, the poles of the system is shifted in left-hand side or the transient response of the system is...

06 February 2018 4,396 3 View

How can we correlate CV , GCD and EIS?

if, i am getting specific capacitance 151 F/g at 1 A/g in GCD then what will be the appropriate scan rate in CV to get same specific capacitance at potential window 0.9 V for both? How EIS depends...

10 December 2017 8,677 5 View

How can I specify a path and make an object move along a path ?

Hello there! I have three parts in my model: a heat source (a circular coil with a hole in the center), a plate and a an air domain which are merged as a single part. I have carried out an...

09 May 2017 7,509 3 View

Which solvent is suitable to take ICP of NVP cathode material for sodium ion batteries?

I am working on the NVP cathode material for sodium ion batteries. I want to take ICP of that material to get information on chemical composition. I don't know which solvent is suitable for that.

09 February 2017 3,996 2 View

Which binder and electrolyte is good for cathode material for sodium-ion batteries?

29 November 2016 8,145 2 View

Strong and extreme winds in the Arctic?

Hello everybody! Looking for a good source of information on strong and extreme winds in the Arctic (monthly values, annual extremes etc.). The form of maps (with isolines) will be most...

25 January 2021 8,984 4 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View

How to make open-top chambers?

Hi, I wonder if someone would share experience with building open-top chambers for surface heating of soil. My main question is what material can be used to build them? I found researchers using...

05 January 2021 5,396 6 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

Inconsistent results after plasmid re-purification from frozen stock?

Hello everyone, I am performing reporter gene assays on some uORF mutants vs my WT 5' UTR of my gene of interest. I am doing double transfection on HEK293T cells, using Nanoluc as a test reporter...

22 November 2020 2,746 5 View

Does anyone know of a good review or comparison article on battery performance in cold (arctic) conditions?

I am starting a project to compare different meteorological sensors for austere sites (no power, and little solar or wind availability). I know battery performance is a topic I will have to...

13 November 2020 7,576 3 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View

RS dataset of ice cover of Arctic Antarctic?

I am looking for datasets of ice cover of Arctic Antarctic. I want only India related dataset. If you have any source of dataset please share with me. It will be very helpful for my...

13 October 2020 7,137 1 View