Do I need a Biosafety facility for just isolating DNA/RNA/microRNA from blood of a TB or cancer patient? Please note that I will not be culturing the cells.
Well in my own opinion, I would recommend that you do your extractions in a Biosafety facility. Please review this document. You will see that the work you have described may fall under the BSL-2 category. Please refer to http://www.cdc.gov/biosafety/publications/bmbl5/bmbl5_sect_iii.pdf. As a matter of rule, it is important to adhere strictly to these regulations irrespective of how superiors or entire group might disregard it. Integrity is key at the end . I hope this helps.
Ideally, TB being a communicable disease, pose a threat on the researcher. Bio-safety facility ensures the safety and prevents contamination as well. DNA/RNA/miRNA are molecular component of the cell and prone to external contamination as microbes can easily contaminate it. Cancer, although non-communicable, but impose threat on molecular extraction.
But is it mandatory to have a bio-safety facility for such laboratory investigations? For example according to DBT guidlines?
Well in my own opinion, I would recommend that you do your extractions in a Biosafety facility. Please review this document. You will see that the work you have described may fall under the BSL-2 category. Please refer to http://www.cdc.gov/biosafety/publications/bmbl5/bmbl5_sect_iii.pdf. As a matter of rule, it is important to adhere strictly to these regulations irrespective of how superiors or entire group might disregard it. Integrity is key at the end . I hope this helps.
Yes, they do have guidelines to ensure safety of the research scientists and scholars as TB is communicable and cancer is grave disease.
As per the international guideline, for handling TB samples, it require P3 facility if you don't know whether it is Drug Resistance case or not. ? At least Biosafety II is required because its Air born disease. As per the IBSC guidliine Biosafety is Must for handling TB samples in lab. You can refer "Guideline and Handbook for Institutional Biosafety Committees" .
Though it is not required to maintain aseptic condition for DNA extraction. As TB is communicable infectious agent, you should take some extra care for your own mental satisfaction. DNA of TB should be extracted at least in level 2 Bio-safety cabinet.
Tuberculosis is fairly common amongst Indians due to lack of appropriate care. Most Visa applications have a mandatory test for chest examination if you are traveling abroad, considering every third Indian is infected. You must ideally follow the international guidelines as suggested by Maulik Rachh. Pasted here is a link for your information.
http://www.cdc.gov/tb/publications/slidesets/infectionguidelines/d_link_text.htm
http://www.idpas.org/SCNReportSite/supplements/PATH%20supplement%20BloodCollectionManual.pdf
Yes you do. Working with blood and diseases you can be infected so you do.
Working with any human sourced biological materials require biosafety labs at the relevant biosafety levels. It is for your own protection. Please talk to you institutional biosafety office and follow their guidelines.
It depends on your institution, and your state or country - the regulations vary! For example, apparently there are some things which are much more dangerous in California than in Texas. Contact your institution's occupational health or safety office and ask their advice and guidance.
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