Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

What is the name of a man that was able to memorize all the phone books in England?

I remember learning about this man in my neuroscience courses when studying learning and cognition. From what I can remember, he is/was British, on the spectrum, worked for either the post...

21 April 2020 1,133 3 View

DdRAD library preparation- double bands after PCR?

09 March 2020 3,304 1 View

Would whole-proteome iTRAQ analysis be an appropriate method for validating and quantifying silencing activity of artificial miRNAs?

I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...

29 January 2020 9,994 3 View

How long can you store extracted xanthophyll samples?

Samples were extracted following Thayer and Bjorkmann 1990 using 80% cold acetone and were stored in a freezer in HPLC vials. Should I expect degradation of pigments with time?

11 November 2018 7,499 2 View

How can I collect blood from a neonatal mouse?

I would like to collect blood from a 1 day old mouse, terminal collection is fine because I also need to collect tissues. Can this be done by cardiac puncture or what method do people use to...

05 July 2018 4,326 16 View

Alternatives to ImageJ?

Are there more efficient techniques available, rather than ImageJ, for quantifying herbivory damage from photos of leaves? I have a basic understanding of machine learning but don't want to spend...

19 June 2018 3,021 3 View

Supplier for xanthophyll pigment standards?

I am trying to find a supplier in the US to purchase HPLC pigment standards for the xanthophyll cycle pigments, specifically antheraxanthin. I have found a supplier for zeaxanthin and...

31 October 2017 5,902 3 View

Has anyone used Dedoose qualitative analysis software? What did you think of it?

I'm a qualitative researcher not currently attached to a University and so without access to expensive, traditional QDA software. I'm looking for an affordable alternative that my employer won't...

12 October 2017 1,993 7 View

Is there a generally accepted substitution rate for fish mitochondrial DNA? Or, specifically, for ND2?

For divergence time estimation

15 March 2017 2,524 3 View

No title

02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

No title

28 February 2021 9,936 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View