Do human OSKM genes work in rat and mouse cells?

More Rodolfo Goya's questions See All

Δ15N fractioning records have negatives values, what that means it?

Hello I have 94 sediment (2cm slices) from cores obtained in four mangroves drainages of northern ecuadorean coast, that exhibit atypical values of δ15N (negative values) as well as higher...

27 January 2024 8,710 0 View

How can I do a correlational analysis on two different Likert scales?

I use Accessibility Usabaility Scale on Level of Accessibility and Usability for each communication channels (television, radio, social media, government websites, and print media) in obtaining...

18 November 2023 4,477 3 View

Information of Feeding of ecuadorean freshwater fishes?

Hello, maybe somebody could provide me information about the feeding of this freshwater fishes of the ecuadorean coast? Bryconamericus dahli Brycon dentex Brycon sp Chaestostoma Mesoheros...

16 November 2023 2,828 2 View

References on how to comment a quotation in academic paper?

I need references on how to comment a quotation in academic paper and kinds of comments.

26 October 2023 5,364 1 View

Could someone recommend me an easy-to-prepare solution to preserve gelatinous holoplankton?

Ctenophores and other gelatinous holoplankton beings obtained with planktonic meshes burst when adding 70% alcohol

15 July 2023 9,894 0 View

How to eliminate a contamination faint band on negative control?

We performed several PCR assays to detect virulence factor of pathogenic strains, but a faint band amplified on negative control. After this, we have changed all reaction components (one by one)...

26 April 2023 6,640 3 View

Is Epileptic Aura Continua still a valid diagnosis?

I am interested in understanding whether the 2015 Report of the ILAE Task Force on the Definition and Classification of Status Epilepticus continues to be applicable after the revised 2017 ILAE...

20 February 2023 4,383 2 View

What is the best sonication method to obtain highly concentrated graphene oxide water dispersions?

I would like to ask about the best conditions of sonication of graphite oxide in terms of apparatus (probe or bath), time and power in order to effectively exfoliate the graphite oxide without...

29 November 2022 2,767 1 View

Is it possible to determine sexual maturity in bivalve molluscs using ultrasound or echography?

We want to adjust minimum collection sizes of bivalves for pedestrian collection in mangroves

01 November 2022 5,457 2 View

Which is the most appropriate data repository for an European project in the field of engineering?

Nowadays, research data can be published and uploaded in different repositories such as Zenodo, IEEE and Mendeley. Many national RTO and universities also offer this service. The procedure is very...

17 October 2021 6,946 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Are these iPSC colonies?

I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...

07 July 2024 9,034 2 View

What is the anti anaemic potentials of hibiscus sabdariffa on wister rats?

anti anaemic potentials if hibiscus sabdariffa

01 July 2024 8,429 0 View

What is the best method to measure diameter of new vessels in tissue,e.g. spinal cord?

Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...

01 July 2024 9,744 0 View

Which pH range is allowed for subcutaneous pump administration?

Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...

19 June 2024 5,950 1 View

46 kDa band using a p65 (NFkB) antibody on rat tissue? JESS / Western Blot?

Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...

16 June 2024 4,759 0 View

Luca Gentile

Hi Rodolfo, human OSKM work with mouse. I don't know with rat cells, but my guess is yes. For example, Axololt and Medaka Oct4 genes can reprogram human cells (Tapia et al., Nature, 2012)

Rodolfo Goya

@Luca Thank your very much for your response. We will soon try human OSKM genes in rat cells. I also guess that the they will work. Rodolfo