16 July 2023 0 10K Report

Ctenophores and other gelatinous holoplankton beings obtained with planktonic meshes burst when adding 70% alcohol

More Eduardo Rodolfo Rebolledo Monsalve's questions See All
How can I publish my final master project as an article for a scientif journal?

I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.

16 July 2024 7,431 1 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Can anyone share the JCR impact factor for 2024?

Please can someone share the list of journals with impact factor published in 2024?

27 June 2024 2,927 0 View

Is is posible a negative eelative predicted relevance q2 in PLS SEM?

I am working on a paper and need to calculate the q2 value in a model. However, I found that some of the values are negative. I am trying to interpret these negative values. Does anyone have any...

26 June 2024 1,176 0 View

Is there an HSQC sequnce suitable for 13C labelled small molecules?

I work in glycobiology and one of my duties is synthesize short oligosaccharide with, at least, one sugar universally 13C-labelled. Is there any sequence that can help me with characterization,...

28 May 2024 8,039 0 View

Can someone tell me what is this on my petri dish?

Hi! I left some petri dishes with Pseudomonas aeruginosa cultivated in MHI in the fridge (8°C) during some time off, and when I came back, it was like this. This is just one plate out of many, and...

24 May 2024 5,149 3 View

¿Necesito 10 articulos cientificos en el campo de las comunicaciones?

Estos articulos deben tener estas caracteristicas resumir los siguientes aspectos metodológicos: tipo de enfoque de investigación (cuantitativo, cualitativo o mixto), objetivo, hipótesis, muestra...

15 May 2024 820 0 View

National Bibliography used to be an important source for libraries acquisition. I wonder if in your country it remains important for, can you tell me?

Until the early 2000s, the National Bibliography used to be an important source for the development of library collections, mainly for the acquisition of new items. I would like to know if in your...

23 April 2024 5,037 7 View

Could you please advise on the following matter? I need to ask within a questionnaire, the number of days each episode of disease last??

For example: Episode 1 2 days duration Episode 2 4 days Ep 3 and son on Many thanks in advance

13 April 2024 4,735 0 View

Why Virtual or Augmented reality are yet see as risk by some teachers in STEM teaching in develop critical thinking?

VR or AR can be a useful tool to improve the learning experience of students, mainly in STEM topics or contents considering the complexity and abstraction need to think in analitycal mode, focused...

09 April 2024 739 2 View

Similar questions and discussions
Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

What adhesive should I use to secure samples that float to the bottom of the well plate?

I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there...

03 June 2024 7,728 2 View

Problem while making hydrogel from chitosan and gelatin ?

A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...

29 May 2024 7,367 4 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Alginate Gelatin Chitosan hydrogel??

How can prepare Alginate Gelatin Chitosan hydrogel?

05 May 2024 6,229 2 View

Why protein bands are unclear or invisible on SDS PAGE gel after staining?

Hi everyone! I have carried out SDS PAGE analysis many times. In the experiment, protein sample was hydrolyzed gelatin. After staining, only bands of protein marker (standard) were shown, whereas...

24 April 2024 6,444 1 View

How to dissociate zebrafish embryonic stem cells?

Hi all, I'm trying to isolate zebrafish embryonic stem cells after 3 hpf of zebrafish embryo's. I have a couple of questions. - I already tried Pronase to dechorionate, but it is very difficult....

15 April 2024 9,059 0 View

How can we decide the spinning condition when spinning core-shell electrospun fiber?

Dear all, I'm trying to fabricate core-shell electrospun fiber using 5wt%PCL in HFIP as a core solution and 6.5wt%Gelatin in HFIP as a shell solution. Feed rate of core is 0.4 mL/hr (20G) and that...

12 April 2024 7,046 0 View

Why is my GELMA opaque?

I have been trying to synthesize high methacrylation GELMA using the following protocol : Precise Tuning of Facile One-Pot Gelatin Methacryloyl (GelMA... However, I find that my GELMA is very...

24 March 2024 1,070 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close