16 July 2023 0 10K Report

Ctenophores and other gelatinous holoplankton beings obtained with planktonic meshes burst when adding 70% alcohol

More Eduardo Rodolfo Rebolledo Monsalve's questions See All
Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

Why do some primers have transcript variants/unintended templates for different genes?

Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...

05 October 2020 352 3 View

Do I need to design forward and reverse primers within an exon region for QRT-PCR?

Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...

04 October 2020 7,678 7 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

Animals - Special Issue, "Learning Theory Applied to the Welfare of Animals", looking to submit?

Dear Colleagues, Please see the following link and information for more details. If you are thinking about submitting, please feel free to reach out to me directly here or at...

09 September 2020 6,361 1 View

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How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

How to use the Embedded Region constrain on Abaqus CAE?

Hello fellow academics, For my Final Year Project, I am looking into different ways of modelling the interface between PVA and gelatin. I have successfully modeled the interface using the "tied"...

04 February 2021 8,698 1 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View

How can I reduce the contraction between gold and PDMS?

I want to use gold electrode on the PDMS. We used an e-beam evaporator to deposit gold electrode on the PDMS using the recipe Ti 5nm, gold 80nm, right after using e-beam evaporator, there is no...

28 December 2020 8,607 3 View

What can I use to dilute protein for gelatin zymography?

My protein sample is extracted with RIPA + PMSF ( Phenylmethylsulfonyl fluoride). After protein assay, the concentration is too high (>900ug/mL) for direct application for gelatin zymography...

13 December 2020 7,764 3 View

X-Ray Diffraction analysis of ZnIn2S4?

Dear Researchers I have synthesized gelatin stabilized ZnIn2S4 nanocrystals, I have obtained XRD patterns which is different from its bulk cubic and hexagonal phases. I assume there is some shift...

11 December 2020 7,933 4 View

Why my samples stuck in the top of gelatin zymography?

I am trying to detect MMP2 activity through gelatin zymography. I do can see clear bands. But all my bands stuck in the top of gel. however the ladder passed through...also when i run the gel, the...

03 December 2020 310 2 View

What is the best washing procedure for Gelatin - arabic gum capsules?

I am preparing microcapsules from complex coacervation procedure to encapsulate an oil phase. However, filtration to remove the aqueous phase results in a paste of aggregated particles. So I am...

25 October 2020 3,152 3 View

Does anyone have a reliable protocol to assess cell viability in commercially available GelMA (Gelatin methacryloyl) ?

Hello all, I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing...

13 September 2020 9,086 1 View

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