Is it possible to determine sexual maturity in bivalve molluscs using ultrasound or echography?

More Eduardo Rodolfo Rebolledo Monsalve's questions See All

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

Why do some primers have transcript variants/unintended templates for different genes?

Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...

05 October 2020 352 3 View

Do I need to design forward and reverse primers within an exon region for QRT-PCR?

Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...

04 October 2020 7,678 7 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

Animals - Special Issue, "Learning Theory Applied to the Welfare of Animals", looking to submit?

Dear Colleagues, Please see the following link and information for more details. If you are thinking about submitting, please feel free to reach out to me directly here or at...

09 September 2020 6,361 1 View

What species (or genus even) of crab is this (bivalve using hermit type)?

Two weeks ago I found a peculiar crab in the intertidal zone in SW Florida. when found, the crab was not utilizing a shell. It was similar shaped to porcelain crabs found in our area, but with...

17 January 2021 1,620 4 View

Which is the importance of fixing (e.g. bivalvia) haemocytes and use anti-clotting solutions in flow cytometry?

I am trying to optimize a flow cytometry method to characterize mytilus spp. haemocytes. In literature I found that someone, to avoid clotting, use anti-clotting solutions or dilute and fix the...

05 November 2020 2,522 4 View

How does the N value be counted for gut content analysis?

I read some articles on mollusc and fish diet analysis. The formula for percentage frequency of occurrence stated above: %F0 = Nei/Ne x 100 and percentage numerical abundance : %NI : Ni/N x...

15 October 2020 4,568 3 View

Any method to dye shells for photography without deteriorating them?

Hi, I need to dye bivalves to bring out the details of the shell and to be able to take good photos. I have heard that there is a gaseous compound that allows this and doesn't damage the samples,...

27 September 2020 1,864 4 View

Does weight of bivalve sample influence the concentration of studied pollutants when analyzing by HPLC/LC-MS?

I have this on-going project research where I have to determine the concentration of Endocrine-disrupting chemicals (EDCs) specifically on 4-octylphenol and 4-nonylphenol on bivalve. 1) I have...

03 February 2020 2,216 1 View

Effects/damage of ocean acidification on bivalves?

Dear researchers, I would much appreciate if you could explain or suggest good research papers regarding the effects of ocean acidification on the chemical composition of the valves of mainly...

31 December 2019 7,776 3 View

Does anyone have even a guess at what type of organism (phylum/class?) the following electron micrographs might show?

We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts....

03 November 2019 2,041 2 View

How to stop shrimp haemocytes from clumping?

Hi Everyone, Does anyone have experience in working with haemocytes of P. vannamei or P. monodon? Could you please suggest me any effective method to prevent cell clumping when taking haemolymph...

09 October 2019 1,457 4 View

How to stop shrimp haemocytes from clumping?

Hi Everyone, Does anyone have experience in working with haemocytes of P. vannamei or P. monodon? Could you please suggest me any effective method to prevent cell clumping when taking haemolymph...

01 October 2019 7,311 3 View

How to measure temperature stress to invertebrates and rates of their predatory mortality in artificial bivalve beds?

Hello everyone ! I am designing multi-factor experiments that manipulate the environment in the absence and presence of ecosystem engineers along a environmental stress gradient. I would like...

26 May 2019 1,339 3 View

Eduardo Rodolfo Rebolledo Monsalve

Tim thank you very much for the paper, we want to try these methods on Anadara tuberculosa as soon as we have results I will send them to you.

Eduardo Rebolledo

Stefano Carboni

Hi Eduardo and Tim, I think this paper could answer your question: "Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging" (https://hal.science/hal-00572529/document). Also available here: Article Individual monitoring of gonad development in the European f...

Cheers