Hi All,
It is recommended by Thermo Scientific to used ExpiSf9 cells as suspension culture. I am wondering if anyone has experience with adherent ExpiSF9 cell culture to produce recombinant proteins! If yes, please share. Thanks!!
Dear Seema
did you mean expi 293?
Sincerelly i never tried to useit in adesion instead suspensions, what it happened to me was failure in electric power of the shaker during the night to cultures in suspension which result in extensive cell death.
expi293 derive from hek293 and therefore is possible that as hek works in adhesion also expi293 can do it but one the facts that make expi able to produce amazing yield of proteins (mgs from a 30 ml transfection) is the high cell density that are able to reach in suspension ( biomass g/l is comparable to e.coli ) and i suspect that this is not possible in adesion and that therefore the yields will be strongly reduced and the cost will not make convenient expi293 respect standard hek293.
if your problem is the lack of an orbital shaker able to mantain CO2 atmosphere, try to fit a cheaper shaker platform inside a normal CO2 incubator for cell culture.
infor design a product specific for this purpose bamed celltron
https://www.infors-ht.com/en/shakers/bench-top-shakers/celltron
but probably you may find more cheap adaptable alternatives in catalogues of generalist suppliers as vwr or sigma Aldrich.
good luck
M anuele
Hi Manuele,
Thanks a lot for sharing your experience with expi. Though I asked about Sf9 cells, your comments on expi(hek), in general, gives an idea about their behaviour in suspension vs. adherent culture. Will look into it.
Thank you once again!
Seema
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