Hi,
I have been using bac-to-bac system for protein production using Sf9 and/or Tni cells. I am just curious to know if it is possible to construct baculovirus based plasmids?
Any suggestion is appreciated.
Thanks a lot!
Seema
For protocol see e.g.
https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-expression/insect-protein-expression/bac-to-bac-baculovirus-expression-system.html?gclid=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE&ef_id=EAIaIQobChMIspSd_aLx5gIVjOJ3Ch0yLQuPEAAYASAAEgIUHvD_BwE:G:s&s_kwcid=AL!3652!3!389478895081!p!!g!!baculovirus
I hope it helps!
Thanks a lot Andreas and Roger!
To be more precise I have been looking for a method to prepare the construct. I have come across the following paper which is of great help.
Article Engineering the transposition-based baculovirus expression v...
Hi All, It is recommended by Thermo Scientific to used ExpiSf9 cells as suspension culture. I am wondering if anyone has experience with adherent ExpiSF9 cell culture to produce recombinant...
01 February 2020 6,884 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View