I have recently isolated presumptive pseudomonas aeruginosa from a pool water sample using membrane filtration on Pseudomonas CN agar. This was set up to be confirmed using CMA agar and nutrient agar. Results came back were oxidase positive, casein hydrolysis positive and colonies appeared pigmented. However no fluorescence was observed under UV light.
I was just wondering if without performing further tests would anyone would say this was confirmed as pseudomonas aeruginosa or is fluorescence a requirement?
Thanks
Thank you for your reply that is great, I appreciate that.
Note: I managed to get the culture set up for identification using the VITEK 2 system and it came back as pseudomonas aeruginosa.
How about doing the now classical 16S and rpoB PCR amplification and sequencing? It is really cheap these days...
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