hello, if DNA sequencing is done on genus specific primes, then the sequencing of amplicon can tel us about the specific specie???
if U= 1435 in mann whitney test, then is it correct?? i mean U value may be in thousands???
09 October 2016 7,779 0 View
i got mann whitney test value U=1402, i am confused about this so large value. this value is right? i mean if value comes in thousands then no need to worry or there is any problem??? please
09 October 2016 2,891 2 View
hello anyone who has studied any article that explains the relationship of body weight with onset of paratuberculosis (Johne disease) in cattle..please suggest
07 August 2016 9,339 0 View
hello, i want to enter qualitative data like yes or No OR positive or Negative in Graph pad prism software but how to enter??? please suggest if anyone knows..
07 August 2016 6,397 1 View
dear all scientists, i am in search of an article that contain information about Bacterial DNA degradation/ survival in soil with effect of different temp, pH, humidity etc...please inform if...
07 August 2016 2,867 2 View
hello, i am doing conventional PCR and i want to do sequencing of my PCR product. please guide me how much volume of amplicon is needed for sequencing?
31 December 2015 5,215 6 View
i am facing problem in real time pcr optimization, i am using Bio-Rad system CFX96. i got different peaks on screen. but could not got sigmoid curve. when i run these samples on gel i got a clear...
11 December 2015 9,068 6 View
i am facing problem in real time PCR optimization. i am using Bio Rad system CFX96. I found Tm of primers 54C in gradient PCR. then i run real time PCR but no peaks are seen but i found clear...
10 November 2015 722 4 View
molecular biology
07 August 2015 4,307 5 View
molecular biology, microbiology, genetics, biotechnology
07 August 2015 6,645 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
How can I select cells in prime editing without fluorescence-activated cell separation?
31 July 2024 8,551 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View