Which companies make bacterial DNA ? i mean for PCR if we need positive control then which companies supply it?

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If in mann whitnet test U value comes in thousands..U=1435, then is it correct??

if U= 1435 in mann whitney test, then is it correct?? i mean U value may be in thousands???

09 October 2016 7,779 0 View

If mann whitney test value falls in thousands then what it means??

i got mann whitney test value U=1402, i am confused about this so large value. this value is right? i mean if value comes in thousands then no need to worry or there is any problem??? please

09 October 2016 2,891 2 View

Is there any association of body weight with paratuberculosis in cattle?

hello anyone who has studied any article that explains the relationship of body weight with onset of paratuberculosis (Johne disease) in cattle..please suggest

07 August 2016 9,339 0 View

How to write qualitative data in Graph pad prism software?

hello, i want to enter qualitative data like yes or No OR positive or Negative in Graph pad prism software but how to enter??? please suggest if anyone knows..

07 August 2016 6,397 1 View

Is there any research article published that indicate bacterial DNA degradation/survival in soil?

dear all scientists, i am in search of an article that contain information about Bacterial DNA degradation/ survival in soil with effect of different temp, pH, humidity etc...please inform if...

07 August 2016 2,867 2 View

When sequencing PCR products, how much volume of amplicon is required?

hello, i am doing conventional PCR and i want to do sequencing of my PCR product. please guide me how much volume of amplicon is needed for sequencing?

31 December 2015 5,215 6 View

DNA sequencing tel you about specie?

hello, if DNA sequencing is done on genus specific primes, then the sequencing of amplicon can tel us about the specific specie???

31 December 2015 8,112 0 View

I am facing problem in optimizing real time PCR...?

i am facing problem in real time pcr optimization, i am using Bio-Rad system CFX96. i got different peaks on screen. but could not got sigmoid curve. when i run these samples on gel i got a clear...

11 December 2015 9,068 6 View

Any advice on the problem in optimization of real time PCR?

i am facing problem in real time PCR optimization. i am using Bio Rad system CFX96. I found Tm of primers 54C in gradient PCR. then i run real time PCR but no peaks are seen but i found clear...

10 November 2015 722 4 View

What are controls in PCR? if we want to get positive control, then how can we get? i mean comercially available? or from where we got?

molecular biology

07 August 2015 4,307 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View