Is there any research article published that indicate bacterial DNA degradation/survival in soil?

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If in mann whitnet test U value comes in thousands..U=1435, then is it correct??

if U= 1435 in mann whitney test, then is it correct?? i mean U value may be in thousands???

09 October 2016 7,779 0 View

If mann whitney test value falls in thousands then what it means??

i got mann whitney test value U=1402, i am confused about this so large value. this value is right? i mean if value comes in thousands then no need to worry or there is any problem??? please

09 October 2016 2,891 2 View

Is there any association of body weight with paratuberculosis in cattle?

hello anyone who has studied any article that explains the relationship of body weight with onset of paratuberculosis (Johne disease) in cattle..please suggest

07 August 2016 9,339 0 View

How to write qualitative data in Graph pad prism software?

hello, i want to enter qualitative data like yes or No OR positive or Negative in Graph pad prism software but how to enter??? please suggest if anyone knows..

07 August 2016 6,397 1 View

When sequencing PCR products, how much volume of amplicon is required?

hello, i am doing conventional PCR and i want to do sequencing of my PCR product. please guide me how much volume of amplicon is needed for sequencing?

31 December 2015 5,215 6 View

DNA sequencing tel you about specie?

hello, if DNA sequencing is done on genus specific primes, then the sequencing of amplicon can tel us about the specific specie???

31 December 2015 8,112 0 View

I am facing problem in optimizing real time PCR...?

i am facing problem in real time pcr optimization, i am using Bio-Rad system CFX96. i got different peaks on screen. but could not got sigmoid curve. when i run these samples on gel i got a clear...

11 December 2015 9,068 6 View

Any advice on the problem in optimization of real time PCR?

i am facing problem in real time PCR optimization. i am using Bio Rad system CFX96. I found Tm of primers 54C in gradient PCR. then i run real time PCR but no peaks are seen but i found clear...

10 November 2015 722 4 View

What are controls in PCR? if we want to get positive control, then how can we get? i mean comercially available? or from where we got?

molecular biology

07 August 2015 4,307 5 View

Which companies make bacterial DNA ? i mean for PCR if we need positive control then which companies supply it?

molecular biology, microbiology, genetics, biotechnology

07 August 2015 6,645 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View