I have extracted DNA by a BigFish auto-extractor (BFMP02R) kit. The concentrations on a nanodrop are acceptable (30-120ng/ul) but the A260/A280 ratio is consistently low (1.2-1.5). The samples are intended for sequencing (may be some chip). Do I need to purify this with ethanol or will it go with sequencing in this shape? Or if can I change something in the wash or the lysis step? Add/remove something?
Thank you all in advance