Muhammad Zakria

7 Questions 8 Answers 0 Followers

Questions related from Muhammad Zakria

RNA quantification and A260/A280 ratio?

I have been extracting RNA via Qiagen minikit RNA extraction kit, and the conc are looking good on nanodrop (colibri) but the A260/A280 ratios are consistently shown high, in ranges of 3.6 or...

09 November 2021 5,083 12 View

How do you convert a batch of AB1 files to FASTA?

Hi everyone, I have a sequencing data directly from the Seqstuio in AB1 format, which I can visualize in different programs but I convert each AB1 file individually to FASTA format. Previously...

09 October 2021 1,705 5 View

Why Allele Specific Primers give only one allele results while minor allele is not amplified?

I am using ARMS PCR primers, have tried it 1. All four Primers together, 2, outer primers + inner for one Allele and 3. only one outer primer with corresponding outer primer. 1. Control primers...

10 July 2021 5,020 2 View

Thermal Cycler block temperature error, where is the problem?

I am working on this Labnet gradient Thermal cylcer (Multigene optimax) and facing some problem, as when I set a reaction, start it, after some time (mostly after denaturation or in the cycles)...

30 November 2019 3,332 1 View

What is the best drug extraction, plasma deproteinization method for hplc?

We are working on pasma level of Rosuvastatin in hyperlipidemic patients. we tried deproteinization methods e-g with ACN, ethanol, and diethylether in one or two step extractions. the problem is...

29 August 2019 7,329 0 View

DNA quantity and quality

I have extracted DNA by a BigFish auto-extractor (BFMP02R) kit. The concentrations on a nanodrop are acceptable (30-120ng/ul) but the A260/A280 ratio is consistently low (1.2-1.5). The samples are...

01 January 1970 6,994 1 View

Publishing the baseline data of a prospective study as a separate cross-sectional one is okay or not

I have enrolled more patients (362 against the samples size 153) due to loss to follow-up. now the baseline data of all patients show some interesting picture and I want to publish it. the...

01 January 1970 7,909 3 View