Dear colleagues,

I want to analyze some dsDNA (approx 110bp), but only have access to a protein gel acrylamide (AA) mix (30% AA in water, 37.5%:1% Acrylamide:bis-Acrylamide). Can I expect to see something after EtBr staining? What percentage should I use? I thought of using standard TAE as gel buffer and running buffer.

Asked the other way round: Why do people use a different AA:bis-AA mixture for DNA gels? Is there a scientific requirement or is it just historically grown like this?

Thanks for your help!