anyone knows by chance, which mammalian cellular structures it is staining?
Just curious. Thanks!
What would you recommend? It should be cheap and of constant / defined composition, comparable to defatted milk powder (NFDM). Thanks!
31 December 2017 4,501 7 View
Dear Colleagues, I'm working on a recombinant protein (isolated from 3T3 mouse fibroblast supernatants) that behaves a little strange in analytics: Purified by IMAC (it has a His6-Tag) and gel...
11 December 2016 7,614 4 View
In homemade colloidal gel stains based on Coomassie Blue G250, 4..12% phosphoric acid are added as last step to promote colloid formation. How does it work: Is it "just" pH or do phosphate...
02 March 2016 10,122 2 View
Dear experts, I am planning a clean-up step in a protein purification process where I actually thought of using Sephacryl S200. In the stock room, I found some Sephadex G200 and thought, I'd give...
11 December 2015 9,917 6 View
Dear Colleagues, Any suggestions for alternatives to cyanoborohydride (in respect of less toxic, cheaper, suitable for aqueous settings)? It's about the reductive amination of a polysaccharide...
02 March 2015 7,047 10 View
Dear Colleagues, peptide synthesis companies offer different purities at different prices.What actually defines the purity? Several things come into my mind: - sequence errors (i.e. usually...
01 February 2015 4,589 7 View
Dear Colleagues, I happened to get a pretty soiled used centrifuge of this type and simply can't get the rotor out for a thorough cleaning of the machine. Of course, the screw has been removed and...
31 December 2014 5,960 9 View
Dear Colleagues, I'm in search of a ELISA to detect Glucagon Like Peptide 1 (GLP-1) in tissue culture supernatants and serum. Requirements: - detects total GLP-1 (active and inactive, N-terminal...
10 November 2014 9,560 2 View
Dear colleagues, Has anyone got a recipe for said buffer/solution to share? I have a secondary antibody conjugated to HRP that I'd like to store at higher dilution in the fridge/freezer. Probably...
07 August 2014 5,845 6 View
Dear colleagues, I have designed a construct for eukaryotic expression. Before I submit it for synthesis, I would like to find out about the possible implications of 3 (unintended) features that...
07 August 2014 3,422 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View