hi

I hope this file will help you.

https://www.ncbi.nlm.nih.gov/pubmed/26079986

https://file.scirp.org/pdf/AE_2015082615372116.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658367/

Good Luck

Hi Dear Reza

Thank you very much

Of course, I've already seen these links (and many others). I'm looking for the people (in this site) who use chelex routinely in their labs. I want to know their experiences about using this resin and the advantages or disadvantages of this method.

Have a nice time.

Dear Aref,

It is been a long time since i used the Chelex protocol for ground beetles. It worked fine for fresh material or material preserved in ethanol. However I remember that after storaging the chelex extracción for relatively long periods at -20ºC the DNA resulted degradated.

here is the Chelex protocol i was using with ground beetles (Spanish version, however the protocol is in one of my papers). In the literatura there are plenty of variants of this protocol

Extracción con Chelex (Walsh et al. 1991): el material fue homogeneizado y resuspendido en 200 ml de tampón de homogeneización (100 mM Tris, 10 mM EDTA, 100 mM NaCl), la digestión se realizó con proteinasa K a una concentración de 0.25 mM a 55 ºC durante dos h. Posteriormente 10 ml de cada homogeneizado se mezclaron con 200 ml de Chelex al 5% y se incubaron a 56ºC durante media h. Esta mezcla se agitó en un vórtex de 5 a 10 s. La proteinasa K se inactivó colocando las muestras a 95 ºC durante 8 min. Las muestras se agitaron de nuevo 5 s. en vortex y se centrifugaron a 13.000 r.p.m durante 5 s. Se usaron 2 ul del sobrenadante para la reacción de PCR.

El sobrante del homogeneizado y de la extracción Chelex se conservó a –20 ºC en el congelador.

If you have any questions please do not hesitate to contact me.

Hi

Thank you very much Dr. Martinez for sharing the protocol.

Have a nice time.