Aref Alikhani

10 Questions 36 Answers 0 Followers

Questions related from Aref Alikhani

LED transilluminators VERSUS UV transilluminators?

We are going to buy a LED transilluminator for our laboratory, but we usually use EtBr, I want to know how accurate these transilluminators are? I mean, can these transilluminators show the...

02 February 2018 10,063 7 View

Some questions around advantages of mtDNA and cox1 gene in DNA barcoding?

Hi I want to know about the reasons of the following statements that are usually mentioned in DNA barcoding or population genetic papers: 1. High mutation rate due to limited repair system (5-10...

01 January 2018 9,651 8 View

What is the importance of GC-content in DNA barcoding?

Hi One of the statistical analysis that usually is done on DNA barcoding sequences is calculating CG-content. I read in some papers that this sequence (COI or cox1) is usually AT rich in insects....

12 December 2017 7,450 5 View

Should I send these PCR products for sequencing?

Hi I amplified a 710-bp fragment of the mitochondrial cytochrome c oxidase sub-unit I gene using Folmer's universal primers (LCO1490 and HCO2198). But I think the product is not good for...

10 October 2017 695 25 View

Stability of Tris/EDTA stock solutions?

Hi I made 100 mL of Tris (1M, pH=8) and EDTA (0.5, pH=8) stock solutions about a month ago and I haven't used them until now, I keep them away from light, in a closet, but when I'm not at the lab...

08 August 2017 6,257 5 View

Does keeping frozen insects at room temperature for some minutes damage their DNA?

Hi I want to take picture from some of the frozen insects that I keep them at minus 20, so I have to keep them at room temperature for some minutes. I'm afraid of DNA degradation, of course I did...

07 July 2017 3,572 3 View

Pigment contamination in DNA

As I said here: https://www.researchgate.net/post/Looking_for_a_DNA_extraction_protocol_from_adult_beetles_Coleoptera I finally extracted the DNA from adult beetles using a CTAB protocol. But the...

07 July 2017 1,541 8 View

Looking for a DNA extraction protocol from adult beetles (Coleoptera)

Hi everyone I'm looking for a DNA extraction protocol from beetles, actually I started extracting DNA from different families (scarabidae, carabidae, cerambycidae) about 2 months ago but there...

06 June 2017 7,966 12 View

What is this plant?

Hi Would you please tell me what plant it is?

06 June 2017 3,727 6 View

DNA extraction experiences using Chelex 100 from insects (especially coleoptea)

Hi I will be cheerful if you share your experiences about extracting DNA from Insects (especially beetles) using Chelex 100 resin. If it's possible, please share the protocol and details that you...

06 June 2017 4,359 4 View