Hi,

My lab students are trying to extract DNA from whole blood. They are facing the issue of RBCs lysis.

They have tried two different solutions

1- Tri-HCl solution

2- Sucrose + 10mM Tris-HCl + 5mM of MgCl2 in 495 ml of distilled water. pH was adjusted to 7.5. After this, 5 ml of Triton X-100 was added after autoclave.

But RBCs are not lysing. The pellet remains red even after 4-5 washings.

Can you please guide how we can improve our lysis buffer.

Thank you.