18 Questions 14 Answers 0 Followers
Questions related from Misbah Hussain
Hi, My lab students are trying to extract DNA from whole blood. They are facing the issue of RBCs lysis. They have tried two different solutions 1- Tri-HCl solution 2- Sucrose + 10mM Tris-HCl...
29 April 2025 8,732 1 View
I was designing Tri-ARMS-PCR primers for an intronic variant of PUS10 human gene located at minus strand in chromosome 2. When I run in-silico PCR for forward and allelic reverse primer i give...
03 November 2022 3,308 1 View
Hi, I have recently submitted a review article entitled "Role of c-Src and Reactive oxygen species in cardiovascular diseases" in a reputed journal. I have gone through all the technical...
31 October 2022 7,427 4 View
Hi, I want to ask that what kind of human genome related data can be submitted to the genome databases? can we report the frequencies of already known polymorphisms of our population in...
17 August 2022 9,239 1 View
Hi Everyone, I am working on tetra-ARMS-PCR to genotype the rs6387. The size of amplicons is: control 610bp, A allele 281bp and G allele 382bp. The PCR is showing a perfect amplification with...
12 December 2018 7,935 4 View
I am working on mice aorta and I am new to microscopy. I have prepared slide of mice aorta sections and imaged under light microscope (I know its not very good for analysis of aorta sections)....
30 August 2018 8,011 1 View
I have data of drug response (0,1) and genotypes (1,2,3). in genotypes, some catagories have count less than 5. Fisher exact test cannot be applied as the data is 2X3. Which other test will be...
16 August 2018 1,819 11 View
Hi, I extracted DNA from blood samples by organic method with freshly prepared solutions. Dissolved DNA in double distiled deionized water (Like I did for my previous samples). see figure 1 for...
16 September 2017 8,393 6 View
Hi Everyone, I am working with data of SNPs, I want to do logistic regression analysis. In multinomial logistic regression, is it compulsory to choose most common genotype as reference? or I can...
28 August 2017 4,757 8 View
28 August 2017 3,245 4 View
Hi everyone, I am working on case control genetic data. I have data for SNP with genotypes AA, AG and GG. I want to check the effect of these individual genotypes on the disease outcome, which...
07 August 2017 8,518 3 View
Hi, PCR reaction is always mysterious. You never know what you will come up with. I used to prepare PCR reaction mixture in single appendorf and then shift it to PCR tubes. after shifting I add...
24 January 2017 1,926 2 View
I am working on fatty acid profiling via GC-MS. I have protocol (reagents: Methanol, chloroform, water and BF3) for fatty acid extraction from blood plasma. But my original samples are of blood...
19 June 2016 5,958 10 View
I am working on hypertensive patients. I used to collect their serum samples in gel & clot activator vacutainer. but in two of my clotted whole blood samples (total are 100) when i separated...
13 May 2016 927 4 View
I want to derivatize my fatty acids in methyl esters. according to protocol I should add 10% BF3/methanol in the sample. I want to confirm that does 10% BF3/methanl means 10 ml BF3 in 90ml...
03 February 2016 4,366 3 View
I want to analyze Fatty Acid Methyl Esters on GC-MS. Currently i am planning to use 30 m x 0.25 mm-internal diameter 70% cyanopropyl polysilphenylene-siloxane 0.25 μm TR-Fatty Acid Methyl Esters...
06 January 2016 3,593 15 View
Kindly also tell me a bit detail of following derivatization methods 1-Acetyl chloride derivatization 2-HCl derivatization 3- NaOCH3 derivatization 4-BF3 derivatization 5- H2SO4 derivatization
21 November 2015 5,649 5 View
Hi Everyone, I want to design ARMS PCR primers to genotype rs4762 (C-->T). But the problem is GC content. overall GC content of Sequence is 56% but the region near SNP is GC rich. I tried to...
01 January 1970 6,403 6 View
