How it's possible to extract DNA from cotton oil? Could you give me some useful protocols?
Thank you in advance!
Pure cotton oil should have very little DNA if any. Since DNA is hydrophylic, you can try to extract by adding water, vortex multiple times and then extract DNA from the aqueous layer. Classical gDNA purification from there after. Will be enough for PCR only.
Hi! Check the following link. I believe it is cottonseed oil.
http://journal.cricaas.com.cn/ch/cottonsci/2009/02/cs090212.pdf
It is in Chinese language, go to Google translate and translate to the language you want.
Good Luck!!!
It seems that translation is not so easy.
If there are any other advices, please let me know.
Hi Bakhtiyor,
Most of the DNA would be in the non-oily part of the cotton seed.The question is - what do you need your DNA for? If it is for PCR - you might be lucky, otherwise you cannot extract large amounts from oil. Saying that - if you have the resources - you can buy yourself a kit for DNA extraction from oily tissues, such as brain and start directly with the oil. Otherwise I would do 10:1 oil to water - homogenise by vortexing or pipetting, then centrifuge and take the water phase, from which I would try to extract with any standard gDNA method. When you are at the final step - DNA precipitation, please add some polyacryl carrier (2 microL) per sample, because if the DNA is too diluted it might not precipitate. Last but not least (only if you need it for PCR) - if you feel a bit lazy you can try to avoid DNA purification at all - do your 10:1 extract and then use the water phase directly for PCR... you may be lucky and get what you want.
@Bhakhtiyor seems the Chinese document is scanned one unable to copy the text to check the translation. Sorry for the inconvenience. Only alternative to catch someone is familiar with that language or check the link provide by Fadaka it may help.
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