How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Are there Q Method studies focused on teacher education candidates perspectives toward technology use and integration?

I am interested in learning more about Q Method within teacher education? I'm currently working with colleagues on a study about teacher education faculty perspectives toward technology...

26 October 2019 5,027 4 View

Is there research demonstrating the invalidity of the concept of the personal clown?

I am interested in research which corroborates findings that show the invalidity of notions of 'personal clown' or 'inner clown'. This research mght, for example: - Suggest evidence for how the...

05 March 2018 1,237 2 View

Is anyone aware of data on wealth distributions in different countries?

Hello, I am looking for data on distribution of wealth/net worth within countries around the world. Something like a frequency distribution of net worth within a population, that would allow me to...

17 February 2018 2,439 4 View

What do immunoglobulin variable region promoters look like?

I am trying to figure out how far the promoter/regulatory region of the immunoglobulin variable regions stretches upstream of the startcodon. I am aware that the conserved octamer sequence...

12 July 2017 4,430 2 View

IgM+ or IgG+ continuous B cell lines with sequenced immunoglobulin genes?

I am looking for an IgM+ or IgG+ continous B cell line, for which the immunoglobulin genes have been sequenced, preferentially the VDJ and VJ rearrangements. The cell line is preferentially from...

13 April 2017 3,803 4 View

What does the genome of hybridomas look like?

Is it correct that a hybridoma has the full intact genome of both the splenocyte and the myeloma to which it is fused? Or do genomic reorganizations take place post fusion? What about the genomic...

19 March 2017 6,529 2 View

VH and VL orientation for ScFv design in chimeric antigen receptor design?

Does the orientation of the VH and VL impact the binding properties of a ScFv when applied in a chimeric antigen receptor? Or are both configurations (VH-Linker-VL-CARbackbone vs....

12 October 2016 5,303 3 View

Defining Load Steps of differing load type within ANSYS Workbench?

Hello! I'm currently performing FE stress analysis of a stepped shaft within ANSYS Workbench with the shaft being individually subjected to axial tension, a bending moment and torsion For...

23 June 2016 213 5 View

Use of glass beads in generating single cell suspension of Mycobacterium tuberculosis?

10 April 2016 7,544 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View