Dear all,
I encapsulate pDNA into my nanoparticles, however, my particles are extremely sensitive to pH changes. For radical pH changes like from 4 to 7.4, they aggregate quickly. It cannot be understood by naked eyes, solution is still clear at pH 7.4 but DLS says that there is some aggregation.
This aggregation can be solved by using ultrasonication, I can easily redisperse my nanoparticles with sonicator in short time periods.
However, I know that sonication shears the pDNA. In other words, pDNA is extremely sensitive to ultrasonication, so I should not use sonication not to damage pDNA.
I do not know what to do now. Do you have any suggestion to redisperse my pDNA loaded nanoparticles without sonication. I will be waiting for your answers.
Sincerely,
Dear Anan Yaghmur,
Thank you for your answer. My particles have +15-30 mV zeta potential at pH 5 and +2-3 mV zeta potential at pH 7.4.
You are right, I decrease repulsion power between my nanoparticles by increasing pH and they aggregate. Nevertheless, when I redisperse them in pH 7.4 with sonicator, they go back to their first state and they are stable for quite long time in pH 7.4. In other words, inherent stability of system is high apart from zeta potential.
You need additional stabilization mechanism. You may try steric stabilization by e.g. nonionic surfactants or water soluble polymers.
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