A warm greeting to dear Experts,

I have encountered challenges in producing chitosan microspheres using the solvent evaporation method of emulsion. Based on literature, I followed the single emulsion approach, but unfortunately, I did not achieve any successful results.

It is important to note that the purity of the materials used has been verified and confirmed.

The strategies I have implemented to facilitate microsphere formation are as follows:

Using synthetic polymer PVA, liquid paraffin, industrial olive oil, industrial canola oil, dichloromethane, and chlorofoam as the organic phase.

Modifying the experimental procedure by adding or removing process steps.

Altering the drying method, including oven drying, vacuum drying, and freeze-drying.

Changing the surfactant type (Span 80 and Tween 20), optimizing surfactant concentration, and modifying the frequency of surfactant addition.

Adjusting the washing method by using industrial acetone, modifying the washing procedure, and changing the number of washing cycles.

Switching to a double emulsion method.

I reckon I'm doing something wrong each time, or I've been neglecting (a) parameter(s) I shouldn't have.

Despite implementing these strategies, no successful outcome has been achieved. I would appreciate any guidance or recommendations to resolve this issue.

Note that if you contribute in to fixing this, if you were willing you'd be named as 3rd Author in this work, so that I appreciate your contribution. we've published several Q1 articles before, thus the cost of your contribution would be paid accordingly.