How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery?

We digested the brain tissue with Trypsin( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension. We...

17 August 2020 6,499 3 View

Anybody aware of any potential Covid19 related psychological database?

I guess there must be some data collected regarding Covid and related to the field of psychology/psychiatry, considering its psychological impact. It might be gathered from the patients, family...

04 July 2020 1,607 3 View

How can I reduce/remove the cytotoxicity of methanol?

Hello everyone, If I want to have several concentrations of a drug by using methanol as a solvent, how can I reduce or remove the toxicity of methanol in the solution? And is it necessary to do...

13 February 2020 6,927 3 View

Any algorithm/R package for Bayesian network causal inference of binary data?

I have a dataset of 150 samples of 60 binary variables. The pcalg binCItest results are not impressive and i can not find a straightforward way to do this using bnlearn or catnet. I appreciate if...

16 January 2020 858 5 View

Why the primers designed for certain virus, cause fluorescent signal in naive mice too?

We designed set of primers for certain virus. Fluorescent signals pops up in naive mice too! It is stable time-dependently. While signals are changing in infected mice time-dependently. Is it...

16 July 2019 510 2 View

I have a number of microscope images with different colors. How I can calculate the percentage of colors in each images?

How I can determine number of graphene layers( AFM,raman spectroscopy,XRD'....)

30 October 2017 9,787 2 View

Some one can suggest me refrence for DYMOLA dynamic modeling for electrical modeling and simulation?

i should develop model for electrochemical storage systems with reference to lithium cells and redox-flow batteries. Models will be developed and simulated through Modelica language(DYMOLA 2017),...

22 January 2017 2,576 3 View

Modeling Nucleate Boiling Using ANSYS FLUENT

20 June 2016 5,466 6 View

How should I use patch function for defining liquid-vapor interface?

Hi. I am working on a simulation of evaporation in a capillary tube.I am simulating evaporation of a water droplet sitting at the end of a capillary tube inside a solid body subjected to heat...

16 June 2016 508 16 View

neuro vision science

I am a researcher in neuro vision science and I want to learn more about this field in this site

05 April 2011 593 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View