Hi all
How can we differentiate in the laboratory an FMD positive result using ELISA or PCR. FMD antibodies positive samples may be because of vaccination or past infection.
Also
How please explain what is the normal procedure for FMD diagnosis.
The extracted/isolated serum you can diagnose through ELISA. In viremia, serected body fluid or virus hosting cell or Tissue, RNA can be used for polymerase reaction.
There is a big difference between what can be done in a research setting in a well funded lab in the developed world given a week or two to make the determination, and real-time detection of virus on a farm or in a stock yard in a third world country. FMD virus was eradicated from much of the world decades ago, and good vaccines are available. But eradication from areas where it remains endemic is not a simple task.
Anyway to provide a useful answer to your question we really need to know if you are talking about spending thousands of dollars on one animal, or if you need a more reasonable approach to a larger problem.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865093/
Paton DJ, Sumption KJ, Charleston B.
Options for control of foot-and-mouth disease: knowledge, capability and policy. Philos Trans R Soc Lond B Biol Sci. 2009 Sep 27;364(1530):2657-67. doi: 10.1098/rstb.2009.0100. Review.
PubMed PMID: 19687036; PubMed Central PMCID: PMC2865093.
Article Development of vaccines toward the global control and eradic...
please, anyone has idea about the optimal parameter for cultivation of BHK cells in Bioreactor ( Agitation, DO, CO2 and PH).
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