Did anyone work to make a Panther analysis for upregulated genes ?

31 December 2016 5,732 1 View

Whether to maintain RNA or Cdna concentration constant with control to measure gene expression level in Real time qPCR.?

Hello I am working on the real time PCR for quantifying gene expression levels. Since the conversion rate of RNA to cDNA depends on reverse transcriptase efficiency which things to be consider for...

11 December 2016 4,413 8 View

Has anyone worked on measuring autophagy induced genes by realtime PCR?

31 December 2014 3,284 9 View

Why deep freeze will generate more heat during the initial installation?

31 December 2014 9,471 0 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

11 December 2014 4,774 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

10 November 2014 6,844 5 View

Will the background caused by mammalian cell lysate matter in ELISA?

Can you tell me whether the background caused by cell lysate matters in giving the signal? wil it be okay if I take lysate cell supernatant after short centrifugation?

10 November 2014 1,354 3 View

Has anyone used autophagy dye from Enzo Life Sciences?

10 November 2014 8,199 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View