Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Hello I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the...

07 August 2018 990 0 View

Did anyone work to make a Panther analysis for upregulated genes ?

31 December 2016 5,732 1 View

Whether to maintain RNA or Cdna concentration constant with control to measure gene expression level in Real time qPCR.?

Hello I am working on the real time PCR for quantifying gene expression levels. Since the conversion rate of RNA to cDNA depends on reverse transcriptase efficiency which things to be consider for...

11 December 2016 4,413 8 View

Has anyone worked on measuring autophagy induced genes by realtime PCR?

31 December 2014 3,284 9 View

Why deep freeze will generate more heat during the initial installation?

31 December 2014 9,471 0 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

11 December 2014 4,774 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

10 November 2014 6,844 5 View

Will the background caused by mammalian cell lysate matter in ELISA?

Can you tell me whether the background caused by cell lysate matters in giving the signal? wil it be okay if I take lysate cell supernatant after short centrifugation?

10 November 2014 1,354 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View