Can we use quantitative gene expression levels without reference dye in qPCR MASTER MIX?

Hello I had a small question regarding the reference dye that incorporated in qPCR master mix of 2x Syber green or Taq Man mix. Generally these reference dyes such as ROX is incorporated for the...

07 August 2018 990 0 View

Did anyone work to make a Panther analysis for upregulated genes ?

31 December 2016 5,732 1 View

Whether to maintain RNA or Cdna concentration constant with control to measure gene expression level in Real time qPCR.?

Hello I am working on the real time PCR for quantifying gene expression levels. Since the conversion rate of RNA to cDNA depends on reverse transcriptase efficiency which things to be consider for...

11 December 2016 4,413 8 View

Has anyone worked on measuring autophagy induced genes by realtime PCR?

31 December 2014 3,284 9 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

11 December 2014 4,774 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

10 November 2014 6,844 5 View

Will the background caused by mammalian cell lysate matter in ELISA?

Can you tell me whether the background caused by cell lysate matters in giving the signal? wil it be okay if I take lysate cell supernatant after short centrifugation?

10 November 2014 1,354 3 View

Has anyone used autophagy dye from Enzo Life Sciences?

10 November 2014 8,199 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View