I'm trying to crystallize a protein and I have got sphere crystal almost in 10 well with different conditions (approx. 10-15 in each well). Could you please suggest me what are these and what to do next with them? pI of my protein is 6.12. And my protein buffer pH was from 7.8 to 8.2.
(I have obtained exactly of what is shown in pictures)
Hi Ritu,
This is not a new topic. Check the link below to the similar question.
Hope it will help you.
George.
https://www.researchgate.net/post/How_can_I_change_the_spherulites_to_other_crystal_form
Are you sure those are protein? The second photo looks like cholesterol or lipid. Always check whether crystals are really made of protein before performing serious optimization.
Hello Matthias,
Amazing ! Thank you very much. Mine were also protein crystals.
@George Minasov and Ho Leung Ng ..Thanks for adding your answers.
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